I've been doing a fish in cycle with 4 guppies using Seachem Prime and Seachem Stability dosed daily, as some people are suggesting is a good way of cycling a tank and keeping the fish safe BUT after observing the fish and speaking to people on here its clear that Prime is troublesome. I have always done fish in cycles on my tanks but as I was out of the hobby for about 10 years I thought I would do some research and see what the current tricks are and I stumbled upon the Prime and Stability method as suggested by some Youtubers. The stability seems fine although it is debatable whether it does anything.
Anyway back to Prime, my observations now are how it deals with oxygen and the overall concern about how long it can deplete oxygen or bind compounds for. Seachem say up to 48 hours, saying up to is always a problem because when do you re-dose?, some people say 24 hours and there is the element of the bottle saying it can be used safely up to 5x times the recommended dose in an emergency but again how long is it active for? no one seems to know.
I had been dosing 1 cap of prime every day to detoxify approx 1ppm of ammonia however 1 cap is for 250l, I have 100l so I should in theory have used only half a cap or less but as I thought I had plenty of aeration I went with it. Over the last 24 hours my guppies have not been as active and have been spending more time near the top although they do still swim around and go down to the bottom etc. I have good flow and have a diffuser fitted with an air pump, they are also eating but not as much so I have stopped with the Prime as recommended to do so by people on here and I have to say that initial thoughts are they are right, I think it has a "totting up" effect to be honest as my fish were absolutely fine for about a week before their behavior changed.
If it binds compounds for up to 48 hours as suggested by Seachem then it can take oxygen too in my opinion for the same amount of time, again Seachem say the oxygen depletion is a short lived side effect lasting no more than an hour but is it, in my opinion if something is "active" in the water for up to 48 hours then it is constantly depleting oxygen and if you keep re-dosing daily like I have been doing then it is clear what is going to happen, and that is only with slightly over the recommended dose I dread to think what would happen if you trusted their 5x times the recommended dose in an emergency.
In closing I do think it detoxifies Ammonia/Nitrite but as it depletes Oxygen too for an undetermined period of time it can't be trusted really.
Also as a separate area of concern is I spilled some on an item of clothing and it has bleached it?? which is very worrying.