Hi there,
So...where to begin? First, ideally you should set up the tank, and let it run at least 24 hours, then, shortly before you head off to the store for your first fish, add TSS to the tank. Within two hours, you should add the fish. Our normal recommendation is to add one small fish per ten gallons of water. However, you CAN fully stock the tank, you just need to keep a close eye on it, and be sure to not complicate matters by overfeeding as well. We recommend this method for African cichlid tanks since it is best to fully stock such a tank from the beginning due to territorial issues. For a novice fish-keeper, we'd recommend the one small fish per ten gallons rule for the first two weeks. Within two weeks, TSS should have fully cycled the tank and they can start slowly adding more fish, one at a time.
In regards to ammonia products, yes, they kill TSS. Any type, whether a chloramines remover or detoxifier, etc, anything that says it locks up ammonia or removes ammonia. Do not add TSS for 24 hours after using such a product, and do not add such a product for at least 7 days after using TSS. {Prime does this.}The bacteria is housed in a special stabilized solution of ammonia, so if you remove/lock up the ammonia, you remove all of the food the bacteria require to live.
If you already have fish, and are having an ammonia issue,
it is best to get the ammonia levels down to below 4.0. 4.0 and higher is just as toxic to TSS as it is to fish. While 2.0 -3.5 ppm ammonia may harm some of the TSS bacteria, it should still have some effect. You may want to do a second dose several days after adding the first one, if you are not seeing the results you want. Keep in mind, these are bacteria, not a chemical, so results are slow to see. Give the product at least 5-7 days.
Best temperature for TSS is between 40 and 80 degrees. Freezing and extreme heat will destroy it. Refrigeration is okay, but not necessary.
{This is in terms of storing not cycling.}
You can test the water any time, but really, you should probably wait at least 48 hours. We expect TSS to start slowly seeding the tank, and making a difference in about that time. You have to have some ammonia occur in the tank to provide the cycle needed, so it will usually create levels or reduce levels to around 1.0-1.5 ppm, and they should stay there for a week to 14 days, and then come down. Sorry, these levels would be for both ammonia and nitrite. These are considered stress, but not toxic, levels, and should not cause any long term damage to the fish.
We recommend waiting two weeks before a water change. Of course, if for some reason, the levels go up to a high level again, we would recommend a change at that point, and another dose. Usually, the hobbyist has done something wrong the first time, in such cases.
Nope, bacteria should not affect the pH.
Hmmm....be sure the levels are not already toxic, shake the container thoroughly, be sure to add the right dosage, do not use an ammonia detoxifying product, and be patient. It won't give you zero readings in an hour, like some folks think. By the way, carbon does not affect it but uv lights do...we get those questions too.
And just an interesting point...the large aquariums, such as the Georgia Aquarium, and the Shedd Aquarium, as well as huge research labs in this country and other countries, also use TSS as well as the saltwater version Bio Spira.
If I have not addressed all of your questions, please let me know.
Regards,
Tetra Care