fishmanphil- This subject has been well covered on this site in the scientific section. Science has cultured and seeded with the proper bacteria for many years. Also, you are mistaken in your previous post as well. If one can add 2 ppm of ammonia and test results in 24 hours show 0 ammonia and 0 nitrite, the tank is usually cycled to accept pretty much a full fish load. One uses the same method to know a tank is cycled however you do it except with fish. Dr Tim's ammonium chloride:
2 oz (59 ml) bottle of reagent grade ammonium chloride for use when fishless cycling. Concentration is 50 mg/L of total ammonia-nitrogen (TAN). Dose 1 drop per gallon of aquarium water.
TAN measures using the nitrogen scale and our test kits use the total ion scale. 2ppm TAN = just under 2.6 ppm on an API test kit.
The more interesting issue today is the potential role played by archaea and their role in the mix.
However, I can assure you both Dr. Tim's One and Only and Tetra's Safe Start do indeed work as advertised. I am happy to point you to the peer reviewed research on this subject.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY,
0099-2240/01/$04.00_0 DOI: 10.1128/AEM.67.12.5791–5800.2001
Dec. 2001, p. 5791–5800 Vol. 67, No. 12
Copyright © 2001, American Society for Microbiology. All Rights Reserved.
Identification of Bacteria Responsible for Ammonia Oxidation
in Freshwater Aquaria
[SIZE=9pt]PAUL C. BURRELL, CAROL M. PHALEN, [/SIZE]
[SIZE=6.5pt]AND [/SIZE]
[SIZE=9pt]TIMOTHY A. HOVANEC*[/SIZE]
[SIZE=9pt]Aquatic Research Laboratory, The Aquaria Group, Moorpark, California 93021[/SIZE]
[SIZE=8pt]Received 1 June 2001/Accepted 25 September 2001[/SIZE]
Read the full study here:
http/www.drtimsaquatics.com/wp-content/files/scientificpapers/hovanecAEM_Dec01.pdf
APPLIED AND ENVIRONMENTAL MICROBIOLOGY,
0099-2240/98/$04.0010
Jan. 1998, p. 258–264 Vol. 64, No. 1
Copyright © 1998, American Society for Microbiology
Nitrospira-Like Bacteria Associated with Nitrite Oxidation
in Freshwater Aquaria
[SIZE=9pt]TIMOTHY A. HOVANEC,[/SIZE]
[SIZE=5.5pt]1,2[/SIZE]
[SIZE=9pt]* LANCE T. TAYLOR,[/SIZE]
[SIZE=5.5pt]1[/SIZE]
[SIZE=9pt]† ANDREW BLAKIS,[/SIZE]
[SIZE=5.5pt]1[/SIZE]
[SIZE=6.5pt]AND [/SIZE]
[SIZE=9pt]EDWARD F. DELONG[/SIZE]
[SIZE=5.5pt]1[/SIZE]
[SIZE=9pt]†[/SIZE]
[SIZE=9pt]Department of Ecology, Evolution and Marine Biology, University of California, Santa Barbara,[/SIZE]
[SIZE=9pt]Santa Barbara, California 93106,[/SIZE][SIZE=5.5pt]1 [/SIZE]
[SIZE=9pt]and Aquaria Inc., Moorpark, California 93021[/SIZE][SIZE=5.5pt]2[/SIZE]
[SIZE=8pt]Received 4 September 1997/Accepted 27 October 1997[/SIZE]
Read the full study here:
http/www.drtimsaquatics.com/wp-content/files/scientificpapers/hovanecAEM_Jan98.pdf
If you want links to papers on the ammonia oxidizing Archaea, I can provide you with 3 that deal specifically with these, one is a master's thesis.
Bacteria can definitely survive in a bottle. You would have to read up on how the bacteria survive periods of starvation (ammonia and/or oxygen) and then recover, I am happy to give you those links as well.
I have used Dr. Tim's product 3 times and it performed as it should have all three times. However, I have far more experience with fishless cycling without bottled bacteria. I have had my own small bio-farm for cycling new filters and keeping a number of cycled filters ready to use for some time.
Finally, here are links to some relevant threads on this subject on this site:
http/www.fishforums.net/index.php?/topic/437193-i-found-new-research-on-tank-bacteria-and-commercial-bacteria-starter-products/
http/www.fishforums.net/index.php?/topic/431791-another-bbacterial-starter-digging-out-the-info/
http/www.fishforums.net/index.php?/topic/414863-the-science-of-bottled-bacteria-for-cycling-aquaria/?view=findpost&p=3491829
http/www.fishforums.net/index.php?/topic/402069-bacterial-starter-research/
http/www.fishforums.net/index.php?/topic/394320-an-open-challenge-to-ianho/
http/www.fishforums.net/index.php?/topic/386023-exchange-w-dr-tim-re-nutrifying-bacteria/
Happy reading