Well you know me, never let it rest. only this time there is one difference, no links. I did hiours worth of research on lab studies regarding assorted fish for assorted things- but either pH and /or TDS stuff figured prominently. Here is what I saw- almost every one used an acclimation period of at least 7 days.
The difference between these studies and everything in this thread is they often dissected fish every day and also took sophisticated physiological measurements. And lo and behold some of the change did not show up until the 5th and 6th day of the acclimation period. Especially changes in genetic components. Its very boring and technical so just read the red parts and i will not provide a link to the study either
"
We examined genomic and nongenomic
changes to gills of zebrafish as they were progressively acclimated from moderately hard freshwater to typical soft water over 7 days and held in soft water for another 7 days. Gills were sampled daily and mRNA expression levels of gill Na+-K+-ATPase (NKA) α1a subunit, epithelium calcium channel (ECaC), carbonic anhydrase-1 and 2 (CA-1, CA-2), Na+/H+ exchanger (NHE-2), V-type proton (H+)-ATPase, and copper transport protein (CTR-1) were quantified by real-time PCR. Changes in enzyme activities of gill NKA were determined and protein levels of NKA and ECaC were quantified by Western blotting. Levels of mRNA for ECaC
increased fourfold after day 6, with an associated increase in ECaC protein levels after 1 wk in soft water. CA-1 and CA-2 exhibited a
1.5- and 6-fold increase in gene expression on days 6 and 5, respectively. Likewise, there was
a fivefold increase in NHE-2 expression after day 6. Surprisingly, CTR-1 mRNA showed
a large transient increase (over threefold) on day 6, while H+-ATPase mRNA did not change."
So apparently true acclimation may take a bit more time than even a several hour drip for a number of factors.
"Zebrafish (
Danio rerio) were purchased from a local pet supply store (PetsMart, Canada) and housed in two 40-liter aquaria in dechlorinated Hamilton tap water, which is
considered moderately hard (Na+ 927 ± 16 μM, Ca2+ 946 ± 11 μM, Mg2+ 422 ± 17 μM, Cu2+ 2.1 ± 0.7 μg/l,
pH 8.3), maintained at 28°C (hard water). The fish were allowed 1 wk to acclimate to the new tanks before experimentation."
then....
"Over a period of 7 days, hard water was progressively removed and replaced with ion-poor reverse-osmosis water (∼
15–20% daily, over a period of 15 minutes), until ion levels in the water were reduced to 115 ± 3 μM Na+, 51 ± 1 μM Ca2+, 26 ± 1 μM Mg2+, 1.8 ± 0.5 μg/l Cu2+;
pH 6.8."
Well dang if they didn't do the actual daily level change pretty fast and then let the fish sit in it for 24 hours before doing it again. Talk about a huge sized drop but a short term drip.
Here is another Hoot, Apparently the seminal research into osmoregulation in fish was done in the 1930s and is apparently pretty much unchanged since. It has been expounded upon and made more detailed due to the new methods that have come along since then.
OK I know I promised, but there is a wealth of info on osmoregulation etc. out there, so, if you are nuts enough to want to look at it, this link should keep you busy for a good month or two:
Teleost fish osmoregulation: what have we learned since August Krogh, Homer Smith, and Ancel Keys (they were the folks from the 1930s)
http://ajpregu.physiology.org/content/295/2/R704.full