I conducted the 50% clean last night as planned. Today I was only going to do about 5-10 litres and a filter clean, but I endeavoured to push on to 50% again as the white spot I referenced on the female Minnow's caudal fin is still there, and there is also a second one on her caudal fin; unsure about her other fins, but definitely keeping an eye out. I'm suspicious that I'm about to experience an outbreak of Ich, and I've upped the aquarium salt to 3g/l or thereabouts - it might be a bit above due to the scales not being exact, nor the volume measurement of my tank minus filter, plants, heating apparatus, algae magnet and airstone. She's still hanging at the surface when the light is off, and the eruption looks like it's healing over or becoming a cyst again.
If this is Ich, then - if I'm not mistaken - it must have arrived via an external route; it can't just exist in the tank and not do anything. There have been no new introductions in over a year and a half, excepting plants and - most recently - snails (of which I'm now keeping an eye out and eliminating when I get the chance). Could it have started in the gills of the female Minnow, causing her to hang at the surface? No idea where the appearance of the eruption/cyst on her right flank, just behind her right gills, factors into this - is it something else, i.e. bacterial, or is it also likely Ich?
As per the white spots on her caudal fin, could it be any other parasite, or a certain bacterial infection? It's not fungal, and I've seen fin rot on a Clown Loach, so I don't believe it's fin rot.
In any case, I have both Esha 2000 and Esha Anti-White Spot and Velvet handy (got the latter many months ago 'just in case'...might have been a wise decision). I've never, ever, had Ich before *touch wood*... I'll have to wait at least 24 hours before dosing as the Prime might interact with them. The manufacturer has warned against using Prime with Esha 2000 in an email, so I can only assume the same problen applies to the Esha Anti-White Spot - waiting 24 hours should ensure the reductive potential of the Prime has been mostly exhausted, if not completely (hopefully completely!).
I hope it's safe to run the Esha Anti-White Spot alongside the aquarium salt treatment. As to the temperature treatment, I could turn the heater on and the temperature up, but as these are White Cloud Mountain Minnows and their maximum recommended temperature is usually 22C, I'm not entirely sure if the temperature treatment would be the best approach or do the Minnows much good.
EDIT: The two white spots do appear to be visible from either side on the caudal fin - unless there are spots either side and the female Minnow is moving too much for me to get a good look and notice that - so I don't quite know what to make of that. Could potentially be fin rot, or still Ich.
EDIT 2: The female Minnow now appears to be hanging at the surface even while the tank light is on.