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I wash my hand with hot water prior to entering my tanks and have never had adverse effects from doing it that way. I think introducing a glove that has who knows what on it (powder, etc,) pose more of a threat than a clean hand.You should try using gloves next time. I use the disposable gloves that you can buy these on ebay or amazon with good results. It's not really safe to work in the tank without gloves anyway...