Not Acceptable
COOLING/FREEZING - There is no evidence that whole body cooling reduces pain or is clinically efficacious when used as an adjunct to physical methods of euthanasia in ectothermic animals. Freezing of un-anesthetized animals is not acceptable as a method of euthanasia. Rapid freezing (in liquid nitrogen) of deeply anesthetized animals is acceptable.
AIR EMBOLISM - Air embolism may be accompanied by convulsions, opisthotonos, and vocalization. If used, it should be done only in anesthetized animals.
BLOW TO THE HEAD - Unacceptable practice for most species.
BURNING - Chemical or thermal burning of an animal is not an acceptable method of euthanasia.
CHLOROFORM - Chloroform is a known hepatotoxin and suspected carcinogen and, therefore, is extremely hazardous to personnel.
CYANIDE - Cyanide poses an extreme danger to personnel and the manner of death is aesthetically objectionable.
DECOMPRESSION - Decompression is unacceptable for euthanasia because of numerous disadvantages. Many chambers are designed to produce decompression at a rate 15 to 60 times faster than that recommended as optimum for animals, resulting in pain and distress attributable to expanding gases trapped in body cavities. Immature animals are tolerant of hypoxia, and longer periods of decompression are required before respiration ceases. Accidental recompression, with recovery of injured animals, can occur. Bleeding, vomiting, convulsions, urination, and defecation, which are aesthetically unpleasant, may develop in unconscious animals.
DROWNING - Drowning is not a means of euthanasia and is inhumane.
EXSANGUINATION - Because of the anxiety associated with extreme hypovolemia, exsanguinations - should be done only in sedated, stunned, or anesthetized animals.
FORMALIN - Direct immersion of an animal into formalin, as a means of euthanasia, is inhumane.
HOUSEHOLD AGENTS - Household products and solvents Acetone, quaternary compounds (including CCl4), laxatives, clove oil, dimethylketone, and quaternary ammonium products*, antacids, and other commercial and household products or solvents are not acceptable agents for euthanasia.
HYPOTHERMIA - Hypothermia is not an appropriate method of euthanasia.
NEUROMUSCULAR BLOCKING AGENTS - When used alone, these drugs all cause respiratory arrest before loss of consciousness, so the animal may perceive pain and distress after it is immobilized. (Nicotine, magnesium sulfate, potassium chloride, all curariform agents)
RAPID FREEZING - Rapid freezing as a sole means of euthanasia is not considered to be humane. If used, animals should be anesthetized prior to freezing.
STRYCHNINE - Strychnine causes violent convulsions and painful muscle contractions.
STUNNING - Stunning may render an animal unconscious, but it is not a method of euthanasia