Before asking the next Q I would like to point out the reason that one cannot simply use a ruler to measure the height of the test tube and then go from there. How much allowance do you build in for the thickness of the glass at the bottom of the tube? Its like calculating tank volume in the USA where the manufacturers measure the outside of the glass to do so. But the water that would fit in the tank is actually based on the inside dimensions. To calculate the proper answer here, if one could trust the volume line, requires a ruler that fits inside of the tube.
Now, in the continental U.S., what is the rarest and most endangered fw fish?