Thanks for those thoughts.
It was my understanding that because Neutral Regulator is basic chemistry and all of the chemicals are naturally occurring, that it would not be problematic.
I will look into your suggestion.
@Byron any clue what caused the sudden death of one of my pairs?
I would not want to guess as to the cause, but I do think that the suggestions that other members have made are all contenders--differing parameters (between the two tanks perhaps), something in the chunk of wood, stress or injury from being moved, or the additives getting into the fish's bloodstream.
All chemical substances, or even "natural" substances like plant-derived additives, are usually detrimental to fish.
@seangee and I have opinioned more than once in threads that the reason we see no disease or "inexplicable" fish deaths is because we do not add any of these substances. I use API Tap Water Conditioner minimally, and rarely a comprehensive liquid fertilizer for the floating plants (not since last December). I respect both Seachem and API as good sources for some products [which I use and recommend], but both also make products that I do not think should ever be dumped into an aquarium. If you look at all the additives Seachem "recommend," and use them, there would scarcely be space left in the tank for fresh water.
I have come to realize that a major problem in this hobby is the lack of understanding many have concerning fish biology. To cite from
The Manual of Fish Health:
Fish are basically fluids living within a fluid medium separated only by a fairly permeable membrane. It is impossible to underestimate how close the relationship is between fish and their environment. What this means is that any change in the properties or state of the water around a fish has an immediate and profound impact on its physiology. In fact, fish are much more vulnerable to the changing state of their environment than are terrestrial animals. However, two factors offset this apparent vulnerability. Clearly, having evolved over millions of years, fish have become superbly adapted to survive in their unique environment and can, to some extent, compensate and adjust to changing conditions. |Also, the natural aquatic environment is generally quite stable.