I did use both bottles, or I thought I did. I did find it very odd (as originally stated) that it was clear. I'll be happy to do it again in a bit,
@PheonixKingZ .
Over the years, the guidelines for Nitrates that I have seen (include the API, which I have in front of me) have said 40 ppm or less is recommended. Now
@Metalhead88 you are saying "40 ppm is very high". What scale do you use to determine "very high"? In addition, oddly enough, the nitrate test says I'm riding around 0 which seems odd to me since it's been a week since my last water change. I was expecting 20-30 ppm.
Again, I'm certainly willing to do things differently - but not if there is no data to show it is actually helpful.
When I had my first aquarium in the early 1960's, most of my fish--well, all of them actually--rarely lived a year. We had no internet, few books on fish (and too expensive for a school child) and it was just accepted that fish didn't live long. I got back into the hobby in the 1980's and found a lot had changed. And there was so much I never knew. In the past decade I have been retired and had the time to research into this. Science has moved forward a lot.
Nothing is more important than water changes for healthy fish. I change 60-70% of the tank volume once each week without fail, and have been doing this for over 20 years now. Clean water is the key to fish health. I think one of the best proofs is the fact that every knowledgeable biologist and ichthyologist in this hobby will advise this. I have read articles that calculate the math behind this. But there is more: water in an aquarium deteriorates quite rapidly. Fish take in water via osmosis through every cell, continually. This water is processed by the kidneys, and urinated back into the tank. The average smallest tetra will urinate 30% of its body weight every day. The tank water quickly becomes old stale water. There are other pollutants too. Fish release chemical signals, pheromones and allomones, that allow them to communicate; these remain in the tank until the water is removed, as no filter nor plants can handle them. As they accumulate, they stress fish more and more. As does the increase in "stale" water itself. Then there are the liquid pollutants occurring from the breakdown of organics (fish excrement primarily); these too cannot be removed by any filter. All of this not only affects the fish's physiology, it also causes stress. And stress is the direct cause of 95% of all aquarium fish disease.
Consider also that in their natural habitat, fish do not take a second respiration "breath" in the same water; each respiration is in fresh water because of the low ratio of fish to water volume. Weekly partial water changes of at least 50% but preferably 60-70% come as close as we can to this, though still far removed. No amount of filtration can replace water changes. Any balanced aquarium should be able to function without a filter. But not without water changes.
Nitrates. Scientific studies on the affect of nitrates have largely been concerned with farm food fish. I have read some of these, and the few concerned with aquarium fish. We now know that all fish are poisoned by nitrates; but unlike ammonia and nitrite poisoning which is rapid, nitrates are slower to actually kill the fish. But they continually weaken the fish, affecting its physiology and metabolism, immune system, causing stress...all negative things leading to reduced health and death in time. The level of nitrate, the length of the exposure, and the individual species, all factor in, but one thing is certain: in time nitrate will kill fish. Death may likely come from other things, due to the fish having been weakened by the nitrates.
There are professional ichthyologists and biologists and microbiologists contributing to this hobby. Dr. Neale Monks is one of the most respected, and when he writes about fish everyone should take notice. I know Neale personally online, and have gone to him a few times with my own problems and questions. On nitrates, he told me that 20 ppm was the upper limit, and keeping nitrate as far below this as possible would inevitably improve the fish's health. Cichlids, thought a pretty tough fish, are now known to be seriously impacted by nitrate at 20 ppm; over on the cichlid site they are now suggesting that problems like hole in the head and Malawi bloat may have high nitrates behind them. Much more scientific study is needed, but all now agree that keeping nitrates as low as possible will always improve fish health long-term.
Another thing I have learned is the incredible unique complexity of fish physiology. Fish unlike any terrestrial animals have an incredibly involved physiology that is tied to every aspect of their environment because it is aquatic. Every substance in the water is pulled into the fish's bloodstream; water parameters are in a very narrow range and each freshwater species has evolved to function in this range and experiences varying levels of difficulty if it changes. An aquarium is an artificial environment, but nonetheless every aspect of life within the tank is governed solely by natural processes including fish biology, water chemistry, and aquatic microbiology.
I have so much reference material accumulated that it takes me some time to dig through for this or that, but I will have a dig later on, and post (if I remember...at 70 I do tend to forget now and then, or as I like to think of it, just get side-tracked
).