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Can anyone suggest what wrong?

Hi, Pixie. This can be a bit overwhelming, but you're being given some good advice here. Don't feel bad--most of us have been there, and we're here to help--but listen up and learn, learn, learn!

As for stocking: There's a website called AQadvisor. Check it out. Experienced aquarists can often get away with stocking more than what it says, but for beginners it's right on the money. It will warn you about common incompatibility and school size issues, too.

Right now, your tank is very overstocked, and you have some compatibility issues. If it were me, I would find new homes for the gouramis, the platies, and the harlies for starters. Then play around with aqadvisor until you get your species compatible and your stocking level well under 100%. You have a really pretty setup, and it can be saved!

Different fish have different personalities and different needs, and if you pick them purely on looks or local pet store advice, you're going to end up with some incompatible species and/or wrong conditions.

Forgive me if you mentioned this, but are you testing for ammonia? Also, what do you mean by "practically nothing" for nitrites? Any measurable amount of nitrite is toxic to fish. It sounds like you added a lot of stock to an uncycled filter, and that's part of your problem.
Hi - thanks for your reply! I use a JBL chemical testing kit. No2, No3 and PH are all in the green section on the chart and I test every other day. My water is at 25 degrees. Water change 20/30% on a weekly basis. Fed every evening but not too much. I’ve attached picture too.
 

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what is denetrol?

the harlequin has a genetic defect (bent spine) and it is not from TB.

if you have a cycled aquarium, just move half the filter media into the new tank for an instant cycled tank.

hi - it’s made by JBL. I was told by my shop that I should put a bottle in before introducing fish to help cycle a new tank. Pic attached.
 

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