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I was told only to use those products with fish in the tank because the new bacteria that is introduced will die. Witch makes sense because bacteria is a living organism and needs to feed. If that is the case then your fish less test will continually have horrible reading because the product is no longer present.If you look at all of API's testing it was performed with fish and all of the other similar products testing was done the same. So I'm thinking there is some truth to the bacteria dieing with out fish being present in the start up.
I was told only to use those products with fish in the tank because the new bacteria that is introduced will die. Witch makes sense because bacteria is a living organism and needs to feed. If that is the case then your fish less test will continually have horrible reading because the product is no longer present.If you look at all of API's testing it was performed with fish and all of the other similar products testing was done the same. So I'm thinking there is some truth to the bacteria dieing with out fish being present in the start up.
All have to say is three black guppies(Poecilia reticulate)approximately 1" (2.5)cm in length where used in all of the testing so you did not research to hard
That API study is not scientific nor published anywhere except their site. I do not know when they added the link to the pdf to their site but I did not see when I went there a few months ago. However, the presentation in that pdf is what I would expect from a high school level report.
The sort of things you find here would be the kind of science one should see http/www.mendeley.com/research/improved-nitrifying-enrichment-remove-ammonium-nitrite-freshwater-aquaria-systems/
But here is what I find most interesting in the API studty: "Nitrite removal required an average of 34 days in the untreated Control aquariums and 24 days in the "Quick Start- treated aquariums." Also, why do they not use any media in the filters? (For those folks who keep saying the bacteria is almost all in the filter- note they cycled tanks with gravel but no filter media.)
Now go to Dr. Hovanec's site for One and Only Nitrifying bacteria and you will read that by using his product to cycle a tank without fish and dosing ammonia twice, you have a fully cycled tank in 5-7 days- that means 0 ammonia and 0 nitrite and able to handle a full fish load. But, if you want to use it with fish, it is safe to be adding additional fish in about a week vs API where you need 24 days to 0 out nitrite. Hmmm.
It looks to me like it has given you a start on ammonia processing but you are no where near close to being cycled based on your 4 July readings for nitrite. Anything that helps helps but it would not be safe to add fish to that tank even now after 2 weeks. Keep up with the testing, we may yet find that it has helped a bit because it got your ammonia processing off to a good start.