Well put!, So, I've got to agree with this...
Acclimation for fish getting shipped to me
- Thread starter connorlindeman
- Start date
TwoTankAmin
Fish Connoisseur
The blue text in the abstract was colored by me to emphasize it.
Craig PM, Wood CM, McClelland GB. Gill membrane remodeling with soft-water acclimation in zebrafish (Danio rerio). Physiological Genomics. 2007 Jun;30(1):53-60. DOI: 10.1152/physiolgenomics.00195.2006. PMID: 17299134.
Little is known regarding the ionoregulatory abilities of zebrafish exposed to soft water despite the popularity of this model organism for physiology and aquatic toxicology. We examined genomic and nongenomic changes to gills of zebrafish as they were progressively acclimated from moderately hard freshwater to typical soft water over 7 days and held in soft water for another 7 days. Gills were sampled daily and mRNA expression levels of gill Na(+)-K(+)-ATPase (NKA) alpha1a subunit, epithelium calcium channel (ECaC), carbonic anhydrase-1 and 2 (CA-1, CA-2), Na(+)/H(+) exchanger (NHE-2), V-type proton (H(+))-ATPase, and copper transport protein (CTR-1) were quantified by real-time PCR. Changes in enzyme activities of gill NKA were determined and protein levels of NKA and ECaC were quantified by Western blotting. Levels of mRNA for ECaC increased fourfold after day 6, with an associated increase in ECaC protein levels after 1 wk in soft water. CA-1 and CA-2 exhibited a 1.5- and 6-fold increase in gene expression on days 6 and 5, respectively. Likewise, there was a fivefold increase in NHE-2 expression after day 6. Surprisingly, CTR-1 mRNA showed a large transient increase (over threefold) on day 6, while H(+)-ATPase mRNA did not change. These data demonstrate a high degree of phenotypic plasticity in zebrafish gills exposed to an ion-poor environment. This not only enhances our understanding of ionoregulatory processes in fish but also highlights the need for proper experimental design for studies involving preacclimation to soft water (e.g., metal toxicity).
Full study here https://journals.physiology.org/doi/full/10.1152/physiolgenomics.00195.2006
Basically. they dropped the TDS daily by doing a daily water change: "Over a period of 7 days, hard water was progressively removed and replaced with ion-poor reverse-osmosis water (∼15–20% daily, over a period of 15 minutes), until ion levels in the water were reduced..."
They kept a control tank as well. Every dau they removes a few fish from both tanks, euthanized them and sampled things.
The gist of this is to acclimate the fish to where there are actual internal physiological changes to the fish which adapt them to the new water. So if you want to tell me that you cannot move fish from hard to softm nope. If you want to tell me true acclimatiomn can happen in a matter of minutes or hours, nope.
And if your fw fish have been shipped in a bag for many hours- i.e. 24 or more and you are going to mess with acclimating them, the odds are better you will harm or kill them, imo. Bear in mind that when shipping most fish, how much they alone weigh is almost immaterial. What we are paying to have shipped is mostly water. Decent shippers try to minimize the amount of water and to maximize the amount of air.
However, if you get fish and there are multiple fish in the bag and one or more has died, get the rest of them out of the bag water ASAP. Do not float the bag, do not acclimate the fish. Get them into clean water (dechlored as needed) at a temp. in their normal range ASAP. Make sure it is well oxygenated.
I have sent and received boxes of fish in transit for a day or more with a value in the $1,000s. I plop and drop them and I tell recipients of my fish to do the same. But, this doesn't mean you can put any fish into any water and be fine. Fish have a range of various parameters in which they can live. We shoiuuld never be putting fish into water outside of the range in which they are bred to live.. This means temp. TDS/conductivity, GH and pH. For some fish the are specific other parameters which may be essential for their good health. This often is what is meant when a fish is described as "difficult to keep" or not for "beginners."
Craig PM, Wood CM, McClelland GB. Gill membrane remodeling with soft-water acclimation in zebrafish (Danio rerio). Physiological Genomics. 2007 Jun;30(1):53-60. DOI: 10.1152/physiolgenomics.00195.2006. PMID: 17299134.
Abstract
Little is known regarding the ionoregulatory abilities of zebrafish exposed to soft water despite the popularity of this model organism for physiology and aquatic toxicology. We examined genomic and nongenomic changes to gills of zebrafish as they were progressively acclimated from moderately hard freshwater to typical soft water over 7 days and held in soft water for another 7 days. Gills were sampled daily and mRNA expression levels of gill Na(+)-K(+)-ATPase (NKA) alpha1a subunit, epithelium calcium channel (ECaC), carbonic anhydrase-1 and 2 (CA-1, CA-2), Na(+)/H(+) exchanger (NHE-2), V-type proton (H(+))-ATPase, and copper transport protein (CTR-1) were quantified by real-time PCR. Changes in enzyme activities of gill NKA were determined and protein levels of NKA and ECaC were quantified by Western blotting. Levels of mRNA for ECaC increased fourfold after day 6, with an associated increase in ECaC protein levels after 1 wk in soft water. CA-1 and CA-2 exhibited a 1.5- and 6-fold increase in gene expression on days 6 and 5, respectively. Likewise, there was a fivefold increase in NHE-2 expression after day 6. Surprisingly, CTR-1 mRNA showed a large transient increase (over threefold) on day 6, while H(+)-ATPase mRNA did not change. These data demonstrate a high degree of phenotypic plasticity in zebrafish gills exposed to an ion-poor environment. This not only enhances our understanding of ionoregulatory processes in fish but also highlights the need for proper experimental design for studies involving preacclimation to soft water (e.g., metal toxicity).
Full study here https://journals.physiology.org/doi/full/10.1152/physiolgenomics.00195.2006
Basically. they dropped the TDS daily by doing a daily water change: "Over a period of 7 days, hard water was progressively removed and replaced with ion-poor reverse-osmosis water (∼15–20% daily, over a period of 15 minutes), until ion levels in the water were reduced..."
They kept a control tank as well. Every dau they removes a few fish from both tanks, euthanized them and sampled things.
The gist of this is to acclimate the fish to where there are actual internal physiological changes to the fish which adapt them to the new water. So if you want to tell me that you cannot move fish from hard to softm nope. If you want to tell me true acclimatiomn can happen in a matter of minutes or hours, nope.
And if your fw fish have been shipped in a bag for many hours- i.e. 24 or more and you are going to mess with acclimating them, the odds are better you will harm or kill them, imo. Bear in mind that when shipping most fish, how much they alone weigh is almost immaterial. What we are paying to have shipped is mostly water. Decent shippers try to minimize the amount of water and to maximize the amount of air.
However, if you get fish and there are multiple fish in the bag and one or more has died, get the rest of them out of the bag water ASAP. Do not float the bag, do not acclimate the fish. Get them into clean water (dechlored as needed) at a temp. in their normal range ASAP. Make sure it is well oxygenated.
I have sent and received boxes of fish in transit for a day or more with a value in the $1,000s. I plop and drop them and I tell recipients of my fish to do the same. But, this doesn't mean you can put any fish into any water and be fine. Fish have a range of various parameters in which they can live. We shoiuuld never be putting fish into water outside of the range in which they are bred to live.. This means temp. TDS/conductivity, GH and pH. For some fish the are specific other parameters which may be essential for their good health. This often is what is meant when a fish is described as "difficult to keep" or not for "beginners."
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