Hello again Colin. As always, thanks for checking in with me. You're more knowledgeable than I am, no question. But, here's my take on vacuuming. First, I have a large trough outside and I could never begin to vacuum that 300 gallon trough. So, I just change out a lot of tank water a couple of times a week. This aggressive routine does a couple of things: It removes most of the toxins that dissolve in the water and what toxins that are left are diluted to a safe level in all the new, treated tap water and this keeps my fish in clean water conditions. So, they're healthy and the chances of a healthy fish being infected by some sort of pathogen that probably exits in all tanks, is to me really remote. I treat my large, indoor tanks the same. There's just too much area to vacuum. I change out most of the water every few days, so the fish stay healthy. I really believe that most pathogens aren't going to do well in near pure water conditions. Just my opinion.
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