Never tested for levels, but never noticed any behavioural differences either. If the de-chlor was really going to have an effect then you would soon see my high oxygen dependant fish (large balas) at the surface trying to get some oxygen on board.
I dose to the levels stated on the bottle, though I often can't really be that bothered to work out the volume removed from the tank (especially as it requires taking the level of both sump tanks and the display) so overdose to be on the safe side. If I think I have removed 20 gallons, I will dose for about 25 to make sure.
I think there is chloramine in my water, but as the companies can change so much of the water without telling you, I would rather not risk it relying on a pirece of advice that could be out of date in seconds. That and I don't have the ability to aerate around 100 gallons of water to do my weekly water change.
I guess the reasons no one has answered your experiment is because:
1) O2 test kits aren't common (nor cheap IIRC)
2) No one has reported fish dieing of suffocation just after putting the de-chlor in and therefore any possible effects are too minor to worry about.
I have searched and searched and can find no hint about thiosulphate using up dissolved oxygen when no chlorine is present.
The only articles I can find about overdosing on ST (as one would be by putting it in tank water before replacing water with a direct hose) refer to swimming pools where they state that if you put too much ST in the tub (after overdosing chlorine) then your regular dose of chlorine will be neutralised by the residual chemical:
http/www.rhtubs.com/dichlor_vermonter.htm
That does not sound like the sort of chemical that is going to eat all your oxygen and deplete the levels for your fish if chlorine is not present.