Yeah the other filters will have a certain amount of bacteria in them, but by removeing one you lose a third of your overall bacteria, the other filters will have to reproduce more bacteria to make up the deficit, and that will take time and you will have a mini cycle situation, just dont remove the other filters and i wouldnt gravel vac for a while either, but like i say im not familar with the filtering capicity of the other filters so i dont know if they are enough for your tank, do a google on it and find out the filtering capicity and see if its enough, if not you will have to buy another filter or not remove the internal one.