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This is very useful information for us who want to understand more about filtration and how to have effective filtrations.As mentioned, aerobic bacteria on filter media from a healthy tank will process ammonia into nitrite and nitrite into nitrate (nitrosomonas and nitrospira), but will not oxidize nitrate into nitrogen gas. Very specialized anoxic or anaerobic bacteria are required to do that. I have tried some of the commercial bio-medias that claim to provide micro-pores to culture anaerobic bacteria, and am experimenting with Dr. Kevin Novak's anoxic biocenosis clarification baskets, but beyond floating plants that use ammonia as their nitrogen source and good tank maintenance (including sufficient volume/frequency of partial water changes), I have yet to find a magic bullet for nitrates!
Here's a deep dive into filtration and water quality that you may find helpful.
I'm not sure they're working at all. I have two separate experiments on-going, but I haven't checked nitrates in quite some time... I lack the gumption to do a real controlled experiment with result tracking... Instead, I also have somewhat deep sand, lots of floating water sprite, and do weekly 50%+ partial water changes.I am real curious how your anaerobic bacteria methods are working.