If you are doing a fishless cycle (which I presume is what you mean by "without") then the correct action is to do nothing. Let your beneficial bacteria process it for you until you have 0 ammonia, 0 nitrite, and a spike of nitrate. From this point water change the nitrate out and wait for any more spikes in am, nit. If you see no other spikes then you're tank is ready for a few fish, don't add too many fish all at once because your BB are going to need to compensate for additional waste again. If you add everything at once you will likely get more toxin spikes and kill all your fish.