Hmmm... That's interesting! I don't know if I will try that now but maybe if I do any other cuttings I will. Thanks!Some sources recommend dipping briefly in vinegar when no rooting hormone is available. I haven't had any problem rooting pathos without rooting hormone, but a vinegar dip might help. Try adding a vinegar dipped cutting to your experiment.
I'm going to be moving all future updates to my other 40g thread now... Just so I can keep everything centralized. (https://www.fishforums.net/threads/planted-40g-aquarium-journal.488019/)
I'll do this update for both threads though.
This is day 7 of the pothos cuttings.
From left to right: All tank water (little bit of API leaf zone added), Tank/Tap Water mix (two drops of seachem flourish added to see what would happen yesterday), All Tap Water.