As someone else mentioned, I think it depends a lot on the substrate you have. I have two types of sand layered so never wanted to mix them up, vacuuming was light to get rid of unwanted surface detritus. Now I have a layer of leaves on top of the sand in varying states of decomposition so I only lightly vacuum.
In my previous tank I never had issues at water change and had a black line along the front of the tank about one inch down. However with the recent move I have had a couple of issues with vacuuming the sand and fish ill afterwards. This thread is making me realise it could be because the sand is no longer layered but mixed together (I had to remove it to move the tank and it all got mixed), very fine and fine, and could be trapping bacteria that is being released even with a gentle vacuum.
In my previous tank I never had issues at water change and had a black line along the front of the tank about one inch down. However with the recent move I have had a couple of issues with vacuuming the sand and fish ill afterwards. This thread is making me realise it could be because the sand is no longer layered but mixed together (I had to remove it to move the tank and it all got mixed), very fine and fine, and could be trapping bacteria that is being released even with a gentle vacuum.