The substrate needs to be done again because the parasites are continually reproducing so even if you did it yesterday, there will be more today.
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Loaches and catfish tolerate salt a lot better than Malachite Green or Copper Sulphate, which are the main ingredients in chemical medications used to treat white spot and other external protozoan infections.
As for the dose rates I suggested (2 heaped tablespoons of salt for every 20 litres of tank water), that is safe for all fish, plants, snails, shrimp and filter bacteria.
To work out the volume of water in the tank:
measure length x width x height in cm.
divide by 1000.
= volume in litres.
When you measure the height, measure from the top of the substrate to the top of the water level.
There is a calculator/ converter in the "FishForum.net Calculator" under "Useful Links" at the bottom of this page that will let you convert litres to gallons if you need it.