I agree with Oldman47 but as far as bacteria on the substrate or gravel in a non-UGF tank, any beneficial bacteria on the gravel will definitely be right on the surface as that's the only place there is any water flow. Items nearest to the filter intake would be the ones most likely to house bacteria. I would think that the number would be closer to 10% than 30% though and even if there is 20% elsewhere in the tank, It's unlikely you would be able to move enough items over to make much of a difference. Once you figure how many surfaces (tank glass, plants, decor, filter housings, heaters, substrate, etc.) there are in the tank for bacteria to be on, you would have to move most all the decor to have enough to amount to anything.