I think I can explain the molly problem, at least a major factor. This fish, like all livebearers, must have moderately hard or harder water. This is a subjective term, but GH at 80 (presumably this is in ppm) is much too soft and livebearers will slowly weaken and die because their physiology cannot function without the minerals (calcium and magnesium primarily) in harder water. Fish continually take in water via osmosis through every cell and at the gills, and the substances in the water are filtered out or used, so it is very important what is in the water. That's why I asked about additives, but it also explains why the soft water is slowly killing the livebearers.
Many livebearers will tolerate or manage in softer water at least for a time, but all along they are weakening more and more which leaves them susceptible to other things as well. Platies are a bit less needful compared to mollies, so it is not surprising that the mollies are the first to begin dying.
The gourami is not the same as this is a soft water fish. But fish disease is a very complex subject and I am certainly no expert so I can only note the obvious problems and hopefully other members with more experience with disease can help further.
Ammonia at 0.25 turns up in several threads. Mollies are highly susceptible to ammonia poisoning, not that other fish are not the same but mollies really cannot tolerate ammonia, nitrite or nitrate. Nitrate at 5 ppm is not bad, so don't worry about that. Do you have live plants? Fast growers like floating plants would easily handle the ammonia, and perhaps help with the nitrates.
The Fluval Aqua+ Water Conditioner could be a factor. The website says it contains "Herbal extracts reduce fish stress caused from transportation, handling and acclimatization," without saying what these extracts are; some other similar products contain aloe vera and this is believed to deteriorate fish gills over time, which could be another issue here. The "Coats delicate scales and fins to protect against minor abrasions" is risky too, depending what the additives are. For the future, you might want to consider changing conditioners. I use API's Tap Water Conditioner which does the basics (detoxifies chlorine, chloramine and heavy metals) but nothing else, and so far as I know it contains no questionable additives. It is more expensive per volume, but it is so highly concentrated that you use half as much (or even less) as any other product on the market and that has another benefit of not clogging up fish with chemicals.