is it just the brackets and glue holding it together, or is the wood actually screwed together?
if you have screws in the top holing the legs on, then i think youre fine as far as the vertical pressure. though id be a little concerned about sheering. because the ply on the sides is only connected to the legs and not the base, its not really providing much in the way of crossbracing. but again, its only a 100L tank, and 2x4 is extremely strong. ive built simple 2x4 box stands similar to this for 150g tanks, and it had no problems, so you may just be fine with how it is now.
i would suggest keeping it against a wall though.