How long can the anaerobes survive out of water is a very good question. Often times before the LR gets to it's first destination here in L.A it has been out of water for three to four days. Then the boxes are stacked at the wholesalers. If it is slow, they are put in to tanks the same day. If it is busy it could take a day or two or three, or a week. Then the rock is taken out of the boxes and placed in LR only tanks. These tanks are on there own systems and not fed anything to keep the bacteria's alive.
That's only for those lucky enough to live here in L.A. For the rest of you after the liverock is delivered here, it is sent ground to other wholesalers are LFS's directly from the wholesaler here. That adds another 4 to 5 days out of water.
How often do you think the wholesalers get in new liverock. Well there was a shipment the first week of Dec, then the next one to the U.S. was the first week of Feb, thee has not been another one since. So if your LFS bought LR in late Jan, it was LR that was left to dry for a few days, then placed in a tank for months with no food for the bacteria to consume to stay "live". The next question is also a good one, how long did it sit in your LFS's tank for before they sold it? Is it ina seperate system there as well? Is it fed anything to stay alive?
Theoretically it is possible for the anaerobes to recolonize the core of the LR. Theoretically many things are possible. The recolonization of the anaerobes in the core of the rock just doesn't happen. The best example of this was a study I saw where a piece of dead rock, a piece of Fiji LR, and a piece of rock that was shipped with a coral that later died, were all put into a 120g full reef for three years. They were all in the same tank, they all under went the same conditions. After learning of this theory on LR the scientist took the rocks out, and broke them in half studying the core of the rock with high power microscopes. He saw that the dead rock, had no anaerobes in it's core. The traditional LR, had very little, I believe he used, insignificant amounts, while the rock that came with the coral had full populations.
As he studied the rock further he found that only the surface of the dead rock had grown any type of aerobes bacteria.
This is the really intresting part, the traditional live rock seemed to have a hard layer towards the middle of the rock. His theory was that as the rock was drying the aerobe bacterias retreated with the water towards the center of the rock. Here they mixed with the anaerobes and eventually both colonies died. The hard layer was formed whne the bacteria's died. He believed that when the bacterias died in the rock they clogged the pores of the rock which blocked any future development of the anearobic bacteria. He went on to explain that when the rock is reintorduced into a marine system that the aerobes colonize the surface of the rock so quickly it would make it impossible for the anearobic bacterias to ever get back to the cores of the rock.
In testing the rock that formerly housed corals, he was able to fing huge populations of anaerobes and noticed that the evidene of clogged pores was not present. This is consistent with the rest of the theory becuase these rocks are never removed from water during shipping.
The point you bring up about the anerobic bacteria in the sandbed is a good one, and the next step in the study. Since the theory was shared there is a different scientist who is in the mind state of you guys that the anearobic bacteria could recolonize the core of LR that he is repeating the study, but also burying some pieces of all the types of rock in a DSB to see if a different environment will change the original findings. Unfortanetly we won't get any ifo out of him until IMAC 2007. These kind of studies take time. He is only doing a year I believe.
Anyways it is all intresting to me.
I still think that common sense tells me that if I can buy a piece of LR for $.50 a lbs that has never been out of water, or buy a piece of LR for $3.98 a lbs that has spent extended time out of water, that I would want the cheaper one, if nothing else but to save the $3.48 a lbs for the same type of rock. JMO.