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The dreaded spot on Guppies...

Put the carbon pads in if euthanasia is your goal. I don't think it is, somehow. They are useless anyway - just a marketing thing to keep you replacing them. I haven't used carbon except to remove meds for decades. Velvet kills. Always remember that. It has a serious head start in your tank, and is very dangerous right now.

Plants won't be happy, but should survive a week. You could try 5 days, have a look and be careful.

Do the water change, redose, and do a water change a few days later. It usually takes about 2-3 weeks to beat it completely, though the last time I saw it here I killed it in a week, with no return. The only thing you could have done to help velvet attack would be if you haven't been doing 30% every week. It's a dirty water parasite, and no, dirty water doesn't show on test kits. Overfeeding, not changing water regularly - that's how things like this take off (and what may have happened before they even hit the store). Here, it's when I overfeed young fish.

The tank must be dark. No ambient light. The creature feeds on light.
 
Put the carbon pads in if euthanasia is your goal. I don't think it is, somehow. They are useless anyway - just a marketing thing to keep you replacing them. I haven't used carbon except to remove meds for decades. Velvet kills. Always remember that. It has a serious head start in your tank, and is very dangerous right now.

Plants won't be happy, but should survive a week. You could try 5 days, have a look and be careful.

Do the water change, redose, and do a water change a few days later. It usually takes about 2-3 weeks to beat it completely, though the last time I saw it here I killed it in a week, with no return. The only thing you could have done to help velvet attack would be if you haven't been doing 30% every week. It's a dirty water parasite, and no, dirty water doesn't show on test kits. Overfeeding, not changing water regularly - that's how things like this take off (and what may have happened before they even hit the store). Here, it's when I overfeed young fish.

The tank must be dark. No ambient light. The creature feeds on light.
@GaryE I'll provide updates for the next week or two now. Thank you so much for your help! I'm fully committed to saving these fish, so if the lights are off and towels over the tank then so be it.

Also to the other users who have helped me, thank you so much! I want to ensure moving forward I help other members with the same awesome advise you've given me :) fingers crossed we can fight this!
 
@DeBuGti - Please remember as you go to doublecheck our advice. Look at images of the parasites, etc. We want to help but we don't see what you see in detail.
 
@GaryE

Please see photo of the larger guppy in my tank now with this showing…. Must be velvet?

Other fish species still ok and male guppies still ok. Good new one of the smaller female guppies looks much better now!
 

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Other fish in the tank looking healthy.
 

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